AI Agent Skills

Specialized research workflows for ToolUniverse

ToolUniverse provides AI agent skills that teach agents how to conduct sophisticated scientific research. These skills combine multiple tools into expert-level workflows.

Important

Install all skills with one command:

npx skills add mims-harvard/ToolUniverse

Setup & Configuration

⚙️ Setup: Tooluniverse

Install and configure ToolUniverse for any use case — MCP server (chat-based), CLI (command line with 9 subcommands), or Python SDK (Coding API wit…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/setup-tooluniverse

General Purpose

✨ tooluniverse

Router skill for ToolUniverse tasks. First checks if specialized tooluniverse skills (105+ skills covering disease/drug/target research, gene-disea…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse

Research Skills

✨ create-tooluniverse-skill

Create high-quality ToolUniverse skills following test-driven, implementation-agnostic methodology. Integrates tools from ToolUniverse’s 1,264+ too…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/create-tooluniverse-skill
🧬 Acmg Variant Classification

Systematic ACMG/AMP variant classification using ToolUniverse tools. Given a genetic variant (HGVS, rsID, or gene+change), applies all 28 ACMG crit…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-acmg-variant-classification
✨ Admet Prediction

Comprehensive ADMET (Absorption, Distribution, Metabolism, Excretion, Toxicity) profiling of drug candidates using ADMETAI predictions, SwissADME d…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-admet-prediction
✨ Adverse Event Detection

Detect and analyze adverse drug event signals using FDA FAERS data, drug labels, disproportionality analysis (PRR, ROR, IC), and biomedical evidenc…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-adverse-event-detection
✨ Adverse Outcome Pathway

> Map environmental/industrial chemicals to mechanistic adverse outcome pathways (AOPs) using AOPWiki, quantify toxicological hazard (PubChemTox GH…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-adverse-outcome-pathway
✨ Aging Senescence

Research aging biology, cellular senescence, and longevity using ToolUniverse. Covers senescence markers and pathways, age-related disease genetics…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-aging-senescence
✨ Antibody Engineering

Comprehensive antibody engineering and optimization for therapeutic development. Covers humanization, affinity maturation, developability assessmen…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-antibody-engineering
🔗 Binder Discovery

Discover novel small molecule binders for protein targets using structure-based and ligand-based approaches. Creates actionable reports with candid…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-binder-discovery
✨ Cancer Classification

> Translate free-text tumor descriptions to OncoTree codes, look up cancer subtypes and tissue hierarchies, resolve UMLS/NCI cross-references, and …

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-cancer-classification
✨ Cancer Genomics Tcga

> TCGA/GDC cancer genomics analysis – cohort construction, clinical metadata retrieval, somatic mutation profiling, copy number variation analysis…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-cancer-genomics-tcga
🧬 Cancer Variant Interpretation

Provide comprehensive clinical interpretation of somatic mutations in cancer. Given a gene symbol + variant (e.g., EGFR L858R, BRAF V600E) and opti…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-cancer-variant-interpretation
✨ Cell Line Profiling

Help researchers select and characterize cancer cell lines for experiments. Given a cancer type, gene of interest, or cell line name, profiles mole…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-cell-line-profiling
🧪 Chemical Compound Retrieval

Retrieves chemical compound information from PubChem and ChEMBL with disambiguation, cross-referencing, and quality assessment. Creates comprehensi…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-chemical-compound-retrieval
🧪 Chemical Safety

Comprehensive chemical safety and toxicology assessment integrating ADMET-AI predictions, CTD toxicogenomics, FDA label safety data, DrugBank safet…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-chemical-safety
🧪 Chemical Sourcing

Find commercial sources for chemical compounds using ZINC, Enamine, eMolecules, and Mcule. Covers compound identification, vendor search, pricing, …

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-chemical-sourcing
✨ Clinical Data Integration

Comprehensive drug safety review integrating FDA labels, FAERS adverse event reports, disproportionality analysis, pharmacogenomics, clinical trial…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-clinical-data-integration
✨ Clinical Guidelines

Search and retrieve clinical practice guidelines across 12+ authoritative sources including NICE, WHO, ADA, AHA/ACC, NCCN, SIGN, CPIC, CMA, CTFPHC,…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-clinical-guidelines
✨ Clinical Trial Design

Strategic clinical trial design feasibility assessment using ToolUniverse. Evaluates patient population sizing, biomarker prevalence, endpoint sele…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-clinical-trial-design
✨ Clinical Trial Matching

AI-driven patient-to-trial matching for precision medicine and oncology. Given a patient profile (disease, molecular alterations, stage, prior trea…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-clinical-trial-matching
✨ Comparative Genomics

Cross-species gene and sequence comparison, ortholog analysis, and evolutionary conservation assessment using ToolUniverse tools. Use when comparin…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-comparative-genomics
✨ Computational Biophysics

Solve quantitative problems in biophysics, pharmacokinetics, epidemiology, toxicology, population genetics, and statistical mechanics. Provides rea…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-computational-biophysics
✨ Crispr Screen Analysis

Comprehensive CRISPR screen analysis for functional genomics. Analyze pooled or arrayed CRISPR screens (knockout, activation, interference) to iden…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-crispr-screen-analysis
✨ Custom Tool

> Add custom local tools to ToolUniverse and use them alongside the 1000+ built-in tools. Use this skill when a user wants to: create their own too…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-custom-tool
✨ Data Integration Analysis

Integrate statistical analysis results with biological knowledge from ToolUniverse tools. After computing associations or differential expression, …

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-data-integration-analysis
✨ Data Wrangling

Universal data access reference for scientific research. Teaches how to download bulk data, parse any scientific file format (VCF, h5ad, mzML, PDB,…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-data-wrangling
✨ Dataset Discovery

Find and evaluate research datasets for any scientific question. Teaches how to reason about data needs, search across public repositories, evaluat…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-dataset-discovery
🏥 Disease Research

Generate comprehensive disease research reports using 100+ ToolUniverse tools. Creates a detailed markdown report file and progressively updates it…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-disease-research
💊 Drug Drug Interaction

Comprehensive drug-drug interaction (DDI) prediction and risk assessment. Analyzes interaction mechanisms (CYP450, transporters, pharmacodynamic), …

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-drug-drug-interaction
💊 Drug Mechanism Research

> Drug mechanism of action investigation – systematic strategy to trace a drug from its primary target through pathways to clinical outcomes, iden…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-drug-mechanism-research
💊 Drug Regulatory

> Drug regulatory and approval research – FDA substance registry lookup, drug classification by ATC/EPC/MoA via RxClass, Orange Book generic avail…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-drug-regulatory
💊 Drug Repurposing

Identify drug repurposing candidates using ToolUniverse for target-based, compound-based, and disease-driven strategies. Searches existing drugs fo…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-drug-repurposing
💊 Drug Research

Generates comprehensive drug research reports with compound disambiguation, evidence grading, and mandatory completeness sections. Covers identity,…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-drug-research
💊 Drug Target Validation

Comprehensive computational validation of drug targets for early-stage drug discovery. Evaluates targets across 10 dimensions (disambiguation, dise…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-drug-target-validation
✨ Ecology Biodiversity

Ecology, biodiversity, and conservation biology research — species identification, invasive species assessment, pollinator ecology, population dyna…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-ecology-biodiversity
✨ Electron Microscopy

Search and analyze cryo-EM maps, single particle structures, tomography datasets, and raw micrograph data from EMDB, EMPIAR, and CryoET Data Portal…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-electron-microscopy
✨ Epidemiological Analysis

End-to-end epidemiological data analysis — from research question to statistical report. Covers study design assessment, dataset discovery and down…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-epidemiological-analysis
✨ Epigenomics

Production-ready genomics and epigenomics data processing for BixBench questions. Handles methylation array analysis (CpG filtering, differential m…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-epigenomics
✨ Epigenomics Chromatin

> Epigenomics and chromatin accessibility research – histone modification ChIP-seq data from ENCODE, CTCF binding and chromatin architecture, eQTL…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-epigenomics-chromatin
📊 Expression Data Retrieval

Retrieves gene expression and omics datasets from ArrayExpress and BioStudies with gene disambiguation, experiment quality assessment, and structur…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-expression-data-retrieval
✨ Functional Genomics Screens

Interpret results from CRISPR/shRNA genetic screens using DepMap essentiality data, constraint scores, pathway enrichment, protein networks, drugga…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-functional-genomics-screens
🏥 Gene Disease Association

Find and compare gene-disease associations across multiple databases (DisGeNET, OpenTargets, Monarch Initiative, OMIM, GenCC, Orphanet, ClinVar). P…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-gene-disease-association
✨ Gene Enrichment

Perform comprehensive gene enrichment and pathway analysis using gseapy (ORA and GSEA), PANTHER, STRING, Reactome, and 40+ ToolUniverse tools. Supp…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-gene-enrichment
✨ Gpcr Structural Pharmacology

> Research GPCR receptors, antibody structures, and protein interface analysis using GPCRdb, SAbDab, and PDBePISA. Retrieves receptor families, kno…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-gpcr-structural-pharmacology
💊 Gwas Drug Discovery

Transform GWAS signals into actionable drug targets and repurposing opportunities. Performs locus-to-gene mapping, target druggability assessment, …

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-gwas-drug-discovery
✨ Gwas Finemapping

Identify and prioritize causal variants at GWAS loci using statistical fine-mapping and locus-to-gene predictions. Computes posterior probabilities…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-gwas-finemapping
✨ Gwas Snp Interpretation

Interpret genetic variants (SNPs) from GWAS studies by aggregating evidence from multiple databases (GWAS Catalog, Open Targets Genetics, ClinVar)….

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-gwas-snp-interpretation
✨ Gwas Study Explorer

Compare GWAS studies, perform meta-analyses, and assess replication across cohorts. Integrates NHGRI-EBI GWAS Catalog and Open Targets Genetics to …

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-gwas-study-explorer
✨ Gwas Trait To Gene

Discover genes associated with diseases and traits using GWAS data from the GWAS Catalog (500,000+ associations) and Open Targets Genetics (L2G pre…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-gwas-trait-to-gene
✨ Hla Immunogenomics

Analyze HLA genes, MHC binding, epitope-MHC associations, and immunogenomics for transplant compatibility, vaccine design, and immunotherapy. Integ…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-hla-immunogenomics
✨ Image Analysis

Production-ready microscopy image analysis and quantitative imaging data skill for colony morphometry, cell counting, fluorescence quantification, …

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-image-analysis
✨ Immune Repertoire Analysis

Comprehensive immune repertoire analysis for T-cell and B-cell receptor sequencing data. Analyze TCR/BCR repertoires to assess clonality, diversity…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-immune-repertoire-analysis
✨ Immunology

Immunology research workflows using ToolUniverse tools. Covers antibody-antigen structural analysis (SAbDab, TheraSAbDab), immune protein interacti…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-immunology
✨ Immunotherapy Response Prediction

Predict patient response to immune checkpoint inhibitors (ICIs) using multi-biomarker integration. Given a cancer type, somatic mutations, and opti…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-immunotherapy-response-prediction
🏥 Infectious Disease

Rapid pathogen characterization and drug repurposing analysis for infectious disease outbreaks. Identifies pathogen taxonomy, essential proteins, p…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-infectious-disease
✨ Inorganic Physical Chemistry

Inorganic chemistry, physical chemistry, and materials science — crystal structures, coordination chemistry, bonding theory (covalency, orbital mix…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-inorganic-physical-chemistry
✨ Install Skills

Detect and auto-install missing ToolUniverse research skills by checking common client skill directories and cloning from GitHub if absent. Use whe…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-install-skills
💊 Kegg Disease Drug

> KEGG-based disease-drug-variant research using KEGG Disease, Drug, Network, and Variant databases. Covers disease gene lookup, drug-target analys…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-kegg-disease-drug
✨ Lipidomics

Analyze lipids, lipid metabolism, and lipid-disease associations using LIPID MAPS, HMDB, PubChem, KEGG, and CTD. Covers lipid identification, class…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-lipidomics
📚 Literature Deep Research

Comprehensive literature deep research across any academic domain using 120+ ToolUniverse tools. Conducts subject disambiguation, systematic litera…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-literature-deep-research
✨ Metabolomics

Comprehensive metabolomics research skill for identifying metabolites, analyzing studies, and searching metabolomics databases. Integrates HMDB (22…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-metabolomics
✨ Metabolomics Analysis

Analyze metabolomics data including metabolite identification, quantification, pathway analysis, and metabolic flux. Processes LC-MS, GC-MS, NMR da…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-metabolomics-analysis
✨ Metabolomics Pathway

Metabolomics pathway analysis – metabolite identification, pathway mapping, disease associations, cross-database enrichment, and enzyme/gene linka…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-metabolomics-pathway
✨ Metagenomics Analysis

Analyze microbiome and metagenomics data using MGnify, GTDB, ENA, and literature tools. Search studies by biome/keyword, retrieve taxonomic profile…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-metagenomics-analysis
✨ Microbiome Research

Analyze microbiome and metagenomics data using MGnify, GTDB, ENA, and literature tools. Search studies by biome/keyword, retrieve taxonomic profile…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-microbiome-research
✨ Model Organism Genetics

Cross-species genetic analysis using model organism databases. Maps human genes to orthologs in mouse, fly, worm, zebrafish, yeast, and frog, then …

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-model-organism-genetics
✨ Multi Omics Integration

Integrate and analyze multiple omics datasets (transcriptomics, proteomics, epigenomics, genomics, metabolomics) for systems biology and precision …

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-multi-omics-integration
🏥 Multiomic Disease Characterization

Comprehensive multi-omics disease characterization integrating genomics, transcriptomics, proteomics, pathway, and therapeutic layers for systems-l…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-multiomic-disease-characterization
✨ Network Pharmacology

Construct and analyze compound-target-disease networks for drug repurposing, polypharmacology discovery, and systems pharmacology. Builds multi-lay…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-network-pharmacology
✨ Neuroscience

Neuroscience research and reasoning workflows using ToolUniverse tools. Covers computational neuroscience (rate models, integrate-and-fire neurons,…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-neuroscience
✨ Noncoding Rna

Analyze non-coding RNAs (miRNAs, lncRNAs, circRNAs) using miRBase, LNCipedia, RNAcentral, Rfam, and target prediction databases. Covers ncRNA ident…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-noncoding-rna
✨ Organic Chemistry

Teaches reasoning strategies for organic chemistry problems — reaction product prediction, spectroscopy interpretation, stereochemistry, and quanti…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-organic-chemistry
🏥 Pathway Disease Genetics

Connect GWAS variants to biological pathways for drug target discovery. Maps disease-associated SNPs to causal genes via eQTL colocalization (GTEx)…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-pathway-disease-genetics
✨ Pharmacogenomics

Guide pharmacogenomics (PGx) research – drug-gene interaction lookup, CPIC guideline retrieval, variant-drug annotation, allele function status, F…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-pharmacogenomics
⚠️ Pharmacovigilance

Analyze drug safety signals from FDA adverse event reports, label warnings, and pharmacogenomic data. Calculates disproportionality measures (PRR, …

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-pharmacovigilance
✨ Phylogenetics

Production-ready phylogenetics and sequence analysis skill for alignment processing, tree analysis, and evolutionary metrics. Computes treeness, RC…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-phylogenetics
✨ Plant Genomics

Research plant genes, pathways, and species using PlantReactome, Ensembl Plants, POWO, UniProt, KEGG, and literature tools. Covers plant pathway an…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-plant-genomics
✨ Polygenic Risk Score

Build and interpret polygenic risk scores (PRS) for complex diseases using GWAS summary statistics. Calculates genetic risk profiles, interprets PR…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-polygenic-risk-score
✨ Population Genetics 1000Genomes

> Population genetics research using the 1000 Genomes Project (IGSR) – search populations by superpopulation ancestry (AFR, AMR, EAS, EUR, SAS), r…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-population-genetics-1000genomes
🎗️ Precision Medicine Stratification

Comprehensive patient stratification for precision medicine by integrating genomic, clinical, and therapeutic data. Given a disease/condition, geno…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-precision-medicine-stratification
🎗️ Precision Oncology

Provide actionable treatment recommendations for cancer patients based on molecular profile. Interprets tumor mutations, identifies FDA-approved th…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-precision-oncology
🧬 Protein Interactions

Analyze protein-protein interaction networks using STRING, BioGRID, and SASBDB databases. Maps protein identifiers, retrieves interaction networks …

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-protein-interactions
🧬 Protein Modification Analysis

Analyze post-translational modifications (PTMs) of proteins — modification sites, types, proteoforms, functional effects at PTM sites, and PTM-depe…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-protein-modification-analysis
🧬 Protein Structure Prediction

Predict and analyze protein 3D structure from amino acid sequence using ESMFold and AlphaFold. Covers de novo structure prediction (ESMFold for seq…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-protein-structure-prediction
🧬 Protein Structure Retrieval

Retrieves protein structure data from RCSB PDB, PDBe, and AlphaFold with protein disambiguation, quality assessment, and comprehensive structural p…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-protein-structure-retrieval
🧬 Protein Therapeutic Design

Design novel protein therapeutics (binders, enzymes, scaffolds) using AI-guided de novo design. Uses RFdiffusion for backbone generation, ProteinMP…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-protein-therapeutic-design
✨ Proteomics Analysis

Analyze mass spectrometry proteomics data including protein quantification, differential expression, post-translational modifications (PTMs), and p…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-proteomics-analysis
✨ Proteomics Data Retrieval

> Find and retrieve proteomics datasets from public repositories including MassIVE and ProteomeXchange (which aggregates PRIDE, PeptideAtlas, jPOST…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-proteomics-data-retrieval
🏥 Rare Disease Diagnosis

Provide differential diagnosis for patients with suspected rare diseases based on phenotype and genetic data. Matches symptoms to HPO terms, identi…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-rare-disease-diagnosis
🏥 Rare Disease Genomics

> Rare disease genomics research – disease identification via Orphanet, causative gene discovery, gene-disease validity assessment via GenCC, path…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-rare-disease-genomics
✨ Regulatory Genomics

> Investigate transcription factor binding, cis-regulatory elements, chromatin accessibility, and regulatory variant annotation. Use when asked abo…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-regulatory-genomics
🧬 Regulatory Variant Analysis

Regulatory variant interpretation – GWAS association lookup, eQTL analysis, chromatin state annotation, regulatory element overlap, and trait onto…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-regulatory-variant-analysis
✨ Rnaseq Deseq2

Production-ready RNA-seq differential expression analysis using PyDESeq2. Performs DESeq2 normalization, dispersion estimation, Wald testing, LFC s…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-rnaseq-deseq2
🐍 Sdk

Build AI scientist systems using ToolUniverse Python SDK for scientific research. Use when users need to access 1000++ scientific tools through Pyt…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-sdk
🧬 Sequence Analysis

Retrieve and analyze biological sequences – gene/protein sequences from NCBI, Ensembl, and UniProt. Search nucleotide databases, fetch by accessio…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-sequence-analysis
🧬 Sequence Retrieval

Retrieves biological sequences (DNA, RNA, protein) from NCBI and ENA with gene disambiguation, accession type handling, and comprehensive sequence …

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-sequence-retrieval
✨ Single Cell

“Production-ready single-cell and expression matrix analysis using scanpy, anndata, and scipy. Performs scRNA-seq QC, normalization, PCA, UMAP, Lei…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-single-cell
✨ Small Molecule Discovery

> Find, characterize, and source small molecules for chemical biology and drug discovery. Covers compound identification (PubChem, ChEMBL), structu…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-small-molecule-discovery
✨ Spatial Omics Analysis

Computational analysis framework for spatial multi-omics data integration. Given spatially variable genes (SVGs), spatial domain annotations, tissu…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-spatial-omics-analysis
✨ Spatial Transcriptomics

Analyze spatial transcriptomics data to map gene expression in tissue architecture. Supports 10x Visium, MERFISH, seqFISH, Slide-seq, and imaging-b…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-spatial-transcriptomics
✨ Statistical Modeling

Perform statistical modeling and regression analysis on biomedical datasets. Supports linear regression, logistic regression (binary/ordinal/multin…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-statistical-modeling
✨ Stem Cell Organoid

Research stem cells, iPSCs, organoids, and cell differentiation using ToolUniverse tools. Covers pluripotency marker identification, differentiatio…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-stem-cell-organoid
✨ Structural Proteomics

Integrate structural biology data with proteomics for drug target validation. Retrieves protein structures from PDB (RCSB, PDBe), AlphaFold predict…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-structural-proteomics
🧬 Structural Variant Analysis

Comprehensive structural variant (SV) analysis skill for clinical genomics. Classifies SVs (deletions, duplications, inversions, translocations), a…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-structural-variant-analysis
✨ Systems Biology

Comprehensive systems biology and pathway analysis using multiple pathway databases (Reactome, KEGG, WikiPathways, Pathway Commons, BioModels). Per…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-systems-biology
🎯 Target Research

Gather comprehensive biological target intelligence from 9 parallel research paths covering protein info, structure, interactions, pathways, expres…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-target-research
✨ Toxicology

> Assess chemical and drug toxicity via adverse outcome pathways, real-world adverse event signals, and toxicogenomic evidence. Integrates AOPWiki …

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-toxicology
✨ Vaccine Design

Design and evaluate vaccine candidates using computational immunology tools. Covers epitope prediction (MHC-I/II binding via IEDB), population cove…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-vaccine-design
🧬 Variant Analysis

Production-ready VCF processing, variant annotation, mutation analysis, and structural variant (SV/CNV) interpretation for bioinformatics questions…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-variant-analysis
🧬 Variant Functional Annotation

> Comprehensive functional annotation of protein variants — pathogenicity, population frequency, structural context, and clinical significance. Int…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-variant-functional-annotation
🧬 Variant Interpretation

Systematic clinical variant interpretation from raw variant calls to ACMG-classified recommendations with structural impact analysis. Aggregates ev…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-variant-interpretation
🧬 Variant To Mechanism

> End-to-end variant-to-mechanism analysis: given a genetic variant (rsID or coordinates), trace its functional impact from regulatory context (GWA…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/tooluniverse-variant-to-mechanism

Development Skills

🛠️ DevTU: Auto Discover Apis

Automatically discover life science APIs online, create ToolUniverse tools, validate them, and prepare integration PRs. Performs gap analysis to id…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/devtu-auto-discover-apis
🛠️ DevTU: Code Optimization

> Code quality patterns and guidelines for ToolUniverse tool development. Apply when writing, fixing, or refactoring tool Python code in the ToolUn…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/devtu-code-optimization
🛠️ DevTU: Create Tool

Create new scientific tools for ToolUniverse framework with proper structure, validation, and testing. Use when users need to add tools to ToolUniv…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/devtu-create-tool
🛠️ DevTU: Docs Quality

TOP PRIORITY skill — find and immediately fix or remove every piece of wrong, outdated, or redundant information in ToolUniverse docs. Wrong code, …

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/devtu-docs-quality
🛠️ DevTU: Fix Tool

Fix failing ToolUniverse tools by diagnosing test failures, identifying root causes, implementing fixes, and validating solutions. Use when ToolUni…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/devtu-fix-tool
🛠️ DevTU: Github

GitHub workflow for ToolUniverse - push code safely by moving temp files, activating pre-commit hooks, running tests, and cleaning staged files. Us…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/devtu-github
🛠️ DevTU: Optimize Descriptions

Optimize tool descriptions in ToolUniverse JSON configs for clarity and usability. Reviews descriptions for missing prerequisites, unexpanded abbre…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/devtu-optimize-descriptions
🛠️ DevTU: Optimize Skills

Optimize ToolUniverse skills for better report quality, evidence handling, and user experience. Apply patterns like tool verification, foundation d…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/devtu-optimize-skills
🛠️ DevTU: Self Evolve

> Orchestrate the full ToolUniverse self-improvement cycle: discover APIs, create tools, test with researcher personas, fix issues, optimize skills…

https://github.com/mims-harvard/ToolUniverse/tree/main/skills/devtu-self-evolve

How to Use Skills

After installation, skills are available in your AI coding agent. Some agents activate skills automatically based on your questions, while others may require you to explicitly mention the skill name.

💊 Drug Research

Ask: “Research aspirin comprehensively”

Activates: tooluniverse-drug-research

🎯 Target Analysis

Ask: “Analyze EGFR as a drug target”

Activates: tooluniverse-target-research

🏥 Disease Investigation

Ask: “Generate disease report for diabetes”

Activates: tooluniverse-disease-research

📚 Literature Review

Ask: “Deep literature review on CRISPR”

Activates: tooluniverse-literature-deep-research